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anti-oct4 3/4 n19 polyclonal goat antibody sc-8628  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti-oct4 3/4 n19 polyclonal goat antibody sc-8628
    Anti Oct4 3/4 N19 Polyclonal Goat Antibody Sc 8628, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+anti-oct4+antibody/oct4+antibody/pmc12271868-95-21-29
    Average 90 stars, based on 1 article reviews
    anti-oct4 3/4 n19 polyclonal goat antibody sc-8628 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Purification:

    Article Title: Reversible Disruption of Specific Transcription Factor-DNA Interactions Using CRISPR/Cas9
    Article Snippet: A goat polyclonal anti-Oct4 antibody (N19, sc-8628, Santa Cruz Biotechnology) was used to pull-down Oct4, a rabbit polyclonal anti-HA tag (ab9110, Abcam) was used to pull down dCas9 tagged with HA and mCherry, and a purified polyclonal rabbit anti-Nanog (Nanog Antibody, A300–397A, Bethyl) was used to pull down Nanog.

    Article Title: Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids.
    Article Snippet: • Sucrose (Sigma-Aldrich, cat. no. S7903) • Optimal cutting temperature embedding cryo-embedding matrix (OCT; Thermo Fisher Scientific, cat. no. 12678646) • Dry ice • Triton X-100 (Sigma-Aldrich, cat. no. 11332481001) • Rabbit monoclonal anti-DAZL antibody (Abcam, cat. no. ab215718; RRID: AB_2893177) • Mouse monoclonal anti-RFP (DsRed) antibody (Rockland, cat. no. 00-301-379S; RRID: AB_2611064) • Goat polyclonal anti-OCT4 antibody (Santa Cruz; cat. no. sc-8628; RRID: AB_653551) • Goat polyclonal anti-DDX4 antibody (R&D Systems, cat. no. AF2030; RRID: AB_2277369) • Donkey anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 488 (Thermo Fisher Scientific, cat. no. A-21206; RRID: AB_2535792) ▲ CAutIon Because Alexa Fluor 488 is photosensitive, exposure to light sources should be minimized.

    Article Title: Generation of porcine induced-pluripotent stem cells from Sertoli cells.
    Article Snippet: Accepted Manuscript Generation of porcine induced-pluripotent stem cells from Sertoli cells Piyathip Setthawong, Praopilas Phakdeedindan, Narong Tiptanavattana, Sasitorn Rungarunlert, Mongkol Techakumphu, Theerawat Tharasanit PII: S0093-691X(18)31094-X DOI: 10.1016/j.theriogenology.2018.12.033 Reference: THE 14825 To appear in: Theriogenology Received Date: 15 May 2018 Accepted Date: 20 December 2018 Please cite this article as: Piyathip Setthawong, Praopilas Phakdeedindan, Narong Tiptanavattana, Sasitorn Rungarunlert, Mongkol Techakumphu, Theerawat Tharasanit, Generation of porcine induced-pluripotent stem cells from Sertoli cells, (2018), doi: 10.1016/j.Theriogenology theriogenology.2018.12.033 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

    ChIP-sequencing:

    Article Title: Reversible Disruption of Specific Transcription Factor-DNA Interactions Using CRISPR/Cas9
    Article Snippet: A goat polyclonal anti-Oct4 antibody (N19, sc-8628, Santa Cruz Biotechnology) was used to pull-down Oct4, a rabbit polyclonal anti-HA tag (ab9110, Abcam) was used to pull down dCas9 tagged with HA and mCherry, and a purified polyclonal rabbit anti-Nanog (Nanog Antibody, A300–397A, Bethyl) was used to pull down Nanog.

    Article Title: Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids.
    Article Snippet: • Sucrose (Sigma-Aldrich, cat. no. S7903) • Optimal cutting temperature embedding cryo-embedding matrix (OCT; Thermo Fisher Scientific, cat. no. 12678646) • Dry ice • Triton X-100 (Sigma-Aldrich, cat. no. 11332481001) • Rabbit monoclonal anti-DAZL antibody (Abcam, cat. no. ab215718; RRID: AB_2893177) • Mouse monoclonal anti-RFP (DsRed) antibody (Rockland, cat. no. 00-301-379S; RRID: AB_2611064) • Goat polyclonal anti-OCT4 antibody (Santa Cruz; cat. no. sc-8628; RRID: AB_653551) • Goat polyclonal anti-DDX4 antibody (R&D Systems, cat. no. AF2030; RRID: AB_2277369) • Donkey anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 488 (Thermo Fisher Scientific, cat. no. A-21206; RRID: AB_2535792) ▲ CAutIon Because Alexa Fluor 488 is photosensitive, exposure to light sources should be minimized.

    Article Title: Generation of porcine induced-pluripotent stem cells from Sertoli cells.
    Article Snippet: Accepted Manuscript Generation of porcine induced-pluripotent stem cells from Sertoli cells Piyathip Setthawong, Praopilas Phakdeedindan, Narong Tiptanavattana, Sasitorn Rungarunlert, Mongkol Techakumphu, Theerawat Tharasanit PII: S0093-691X(18)31094-X DOI: 10.1016/j.theriogenology.2018.12.033 Reference: THE 14825 To appear in: Theriogenology Received Date: 15 May 2018 Accepted Date: 20 December 2018 Please cite this article as: Piyathip Setthawong, Praopilas Phakdeedindan, Narong Tiptanavattana, Sasitorn Rungarunlert, Mongkol Techakumphu, Theerawat Tharasanit, Generation of porcine induced-pluripotent stem cells from Sertoli cells, (2018), doi: 10.1016/j.Theriogenology theriogenology.2018.12.033 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

    Sequencing:

    Article Title: Reversible Disruption of Specific Transcription Factor-DNA Interactions Using CRISPR/Cas9
    Article Snippet: A goat polyclonal anti-Oct4 antibody (N19, sc-8628, Santa Cruz Biotechnology) was used to pull-down Oct4, a rabbit polyclonal anti-HA tag (ab9110, Abcam) was used to pull down dCas9 tagged with HA and mCherry, and a purified polyclonal rabbit anti-Nanog (Nanog Antibody, A300–397A, Bethyl) was used to pull down Nanog.

    Article Title: Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids.
    Article Snippet: • Sucrose (Sigma-Aldrich, cat. no. S7903) • Optimal cutting temperature embedding cryo-embedding matrix (OCT; Thermo Fisher Scientific, cat. no. 12678646) • Dry ice • Triton X-100 (Sigma-Aldrich, cat. no. 11332481001) • Rabbit monoclonal anti-DAZL antibody (Abcam, cat. no. ab215718; RRID: AB_2893177) • Mouse monoclonal anti-RFP (DsRed) antibody (Rockland, cat. no. 00-301-379S; RRID: AB_2611064) • Goat polyclonal anti-OCT4 antibody (Santa Cruz; cat. no. sc-8628; RRID: AB_653551) • Goat polyclonal anti-DDX4 antibody (R&D Systems, cat. no. AF2030; RRID: AB_2277369) • Donkey anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 488 (Thermo Fisher Scientific, cat. no. A-21206; RRID: AB_2535792) ▲ CAutIon Because Alexa Fluor 488 is photosensitive, exposure to light sources should be minimized.

    Article Title: Generation of porcine induced-pluripotent stem cells from Sertoli cells.
    Article Snippet: Accepted Manuscript Generation of porcine induced-pluripotent stem cells from Sertoli cells Piyathip Setthawong, Praopilas Phakdeedindan, Narong Tiptanavattana, Sasitorn Rungarunlert, Mongkol Techakumphu, Theerawat Tharasanit PII: S0093-691X(18)31094-X DOI: 10.1016/j.theriogenology.2018.12.033 Reference: THE 14825 To appear in: Theriogenology Received Date: 15 May 2018 Accepted Date: 20 December 2018 Please cite this article as: Piyathip Setthawong, Praopilas Phakdeedindan, Narong Tiptanavattana, Sasitorn Rungarunlert, Mongkol Techakumphu, Theerawat Tharasanit, Generation of porcine induced-pluripotent stem cells from Sertoli cells, (2018), doi: 10.1016/j.Theriogenology theriogenology.2018.12.033 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

    Binding Assay:

    Article Title: Reversible Disruption of Specific Transcription Factor-DNA Interactions Using CRISPR/Cas9
    Article Snippet: A goat polyclonal anti-Oct4 antibody (N19, sc-8628, Santa Cruz Biotechnology) was used to pull-down Oct4, a rabbit polyclonal anti-HA tag (ab9110, Abcam) was used to pull down dCas9 tagged with HA and mCherry, and a purified polyclonal rabbit anti-Nanog (Nanog Antibody, A300–397A, Bethyl) was used to pull down Nanog.

    Article Title: Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids.
    Article Snippet: • Sucrose (Sigma-Aldrich, cat. no. S7903) • Optimal cutting temperature embedding cryo-embedding matrix (OCT; Thermo Fisher Scientific, cat. no. 12678646) • Dry ice • Triton X-100 (Sigma-Aldrich, cat. no. 11332481001) • Rabbit monoclonal anti-DAZL antibody (Abcam, cat. no. ab215718; RRID: AB_2893177) • Mouse monoclonal anti-RFP (DsRed) antibody (Rockland, cat. no. 00-301-379S; RRID: AB_2611064) • Goat polyclonal anti-OCT4 antibody (Santa Cruz; cat. no. sc-8628; RRID: AB_653551) • Goat polyclonal anti-DDX4 antibody (R&D Systems, cat. no. AF2030; RRID: AB_2277369) • Donkey anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 488 (Thermo Fisher Scientific, cat. no. A-21206; RRID: AB_2535792) ▲ CAutIon Because Alexa Fluor 488 is photosensitive, exposure to light sources should be minimized.

    Article Title: Generation of porcine induced-pluripotent stem cells from Sertoli cells.
    Article Snippet: Accepted Manuscript Generation of porcine induced-pluripotent stem cells from Sertoli cells Piyathip Setthawong, Praopilas Phakdeedindan, Narong Tiptanavattana, Sasitorn Rungarunlert, Mongkol Techakumphu, Theerawat Tharasanit PII: S0093-691X(18)31094-X DOI: 10.1016/j.theriogenology.2018.12.033 Reference: THE 14825 To appear in: Theriogenology Received Date: 15 May 2018 Accepted Date: 20 December 2018 Please cite this article as: Piyathip Setthawong, Praopilas Phakdeedindan, Narong Tiptanavattana, Sasitorn Rungarunlert, Mongkol Techakumphu, Theerawat Tharasanit, Generation of porcine induced-pluripotent stem cells from Sertoli cells, (2018), doi: 10.1016/j.Theriogenology theriogenology.2018.12.033 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

    Recombinant:

    Article Title: Reversible Disruption of Specific Transcription Factor-DNA Interactions Using CRISPR/Cas9
    Article Snippet: A goat polyclonal anti-Oct4 antibody (N19, sc-8628, Santa Cruz Biotechnology) was used to pull-down Oct4, a rabbit polyclonal anti-HA tag (ab9110, Abcam) was used to pull down dCas9 tagged with HA and mCherry, and a purified polyclonal rabbit anti-Nanog (Nanog Antibody, A300–397A, Bethyl) was used to pull down Nanog.

    Article Title: Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids.
    Article Snippet: • Sucrose (Sigma-Aldrich, cat. no. S7903) • Optimal cutting temperature embedding cryo-embedding matrix (OCT; Thermo Fisher Scientific, cat. no. 12678646) • Dry ice • Triton X-100 (Sigma-Aldrich, cat. no. 11332481001) • Rabbit monoclonal anti-DAZL antibody (Abcam, cat. no. ab215718; RRID: AB_2893177) • Mouse monoclonal anti-RFP (DsRed) antibody (Rockland, cat. no. 00-301-379S; RRID: AB_2611064) • Goat polyclonal anti-OCT4 antibody (Santa Cruz; cat. no. sc-8628; RRID: AB_653551) • Goat polyclonal anti-DDX4 antibody (R&D Systems, cat. no. AF2030; RRID: AB_2277369) • Donkey anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 488 (Thermo Fisher Scientific, cat. no. A-21206; RRID: AB_2535792) ▲ CAutIon Because Alexa Fluor 488 is photosensitive, exposure to light sources should be minimized.

    Article Title: Generation of porcine induced-pluripotent stem cells from Sertoli cells.
    Article Snippet: Accepted Manuscript Generation of porcine induced-pluripotent stem cells from Sertoli cells Piyathip Setthawong, Praopilas Phakdeedindan, Narong Tiptanavattana, Sasitorn Rungarunlert, Mongkol Techakumphu, Theerawat Tharasanit PII: S0093-691X(18)31094-X DOI: 10.1016/j.theriogenology.2018.12.033 Reference: THE 14825 To appear in: Theriogenology Received Date: 15 May 2018 Accepted Date: 20 December 2018 Please cite this article as: Piyathip Setthawong, Praopilas Phakdeedindan, Narong Tiptanavattana, Sasitorn Rungarunlert, Mongkol Techakumphu, Theerawat Tharasanit, Generation of porcine induced-pluripotent stem cells from Sertoli cells, (2018), doi: 10.1016/j.Theriogenology theriogenology.2018.12.033 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

    Chromatin Immunoprecipitation:

    Article Title: Reversible Disruption of Specific Transcription Factor-DNA Interactions Using CRISPR/Cas9
    Article Snippet: A goat polyclonal anti-Oct4 antibody (N19, sc-8628, Santa Cruz Biotechnology) was used to pull-down Oct4, a rabbit polyclonal anti-HA tag (ab9110, Abcam) was used to pull down dCas9 tagged with HA and mCherry, and a purified polyclonal rabbit anti-Nanog (Nanog Antibody, A300–397A, Bethyl) was used to pull down Nanog.

    Article Title: Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids.
    Article Snippet: • Sucrose (Sigma-Aldrich, cat. no. S7903) • Optimal cutting temperature embedding cryo-embedding matrix (OCT; Thermo Fisher Scientific, cat. no. 12678646) • Dry ice • Triton X-100 (Sigma-Aldrich, cat. no. 11332481001) • Rabbit monoclonal anti-DAZL antibody (Abcam, cat. no. ab215718; RRID: AB_2893177) • Mouse monoclonal anti-RFP (DsRed) antibody (Rockland, cat. no. 00-301-379S; RRID: AB_2611064) • Goat polyclonal anti-OCT4 antibody (Santa Cruz; cat. no. sc-8628; RRID: AB_653551) • Goat polyclonal anti-DDX4 antibody (R&D Systems, cat. no. AF2030; RRID: AB_2277369) • Donkey anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 488 (Thermo Fisher Scientific, cat. no. A-21206; RRID: AB_2535792) ▲ CAutIon Because Alexa Fluor 488 is photosensitive, exposure to light sources should be minimized.

    Article Title: Generation of porcine induced-pluripotent stem cells from Sertoli cells.
    Article Snippet: Accepted Manuscript Generation of porcine induced-pluripotent stem cells from Sertoli cells Piyathip Setthawong, Praopilas Phakdeedindan, Narong Tiptanavattana, Sasitorn Rungarunlert, Mongkol Techakumphu, Theerawat Tharasanit PII: S0093-691X(18)31094-X DOI: 10.1016/j.theriogenology.2018.12.033 Reference: THE 14825 To appear in: Theriogenology Received Date: 15 May 2018 Accepted Date: 20 December 2018 Please cite this article as: Piyathip Setthawong, Praopilas Phakdeedindan, Narong Tiptanavattana, Sasitorn Rungarunlert, Mongkol Techakumphu, Theerawat Tharasanit, Generation of porcine induced-pluripotent stem cells from Sertoli cells, (2018), doi: 10.1016/j.Theriogenology theriogenology.2018.12.033 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

    Clone Assay:

    Article Title: Reversible Disruption of Specific Transcription Factor-DNA Interactions Using CRISPR/Cas9
    Article Snippet: A goat polyclonal anti-Oct4 antibody (N19, sc-8628, Santa Cruz Biotechnology) was used to pull-down Oct4, a rabbit polyclonal anti-HA tag (ab9110, Abcam) was used to pull down dCas9 tagged with HA and mCherry, and a purified polyclonal rabbit anti-Nanog (Nanog Antibody, A300–397A, Bethyl) was used to pull down Nanog.

    Article Title: Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids.
    Article Snippet: • Sucrose (Sigma-Aldrich, cat. no. S7903) • Optimal cutting temperature embedding cryo-embedding matrix (OCT; Thermo Fisher Scientific, cat. no. 12678646) • Dry ice • Triton X-100 (Sigma-Aldrich, cat. no. 11332481001) • Rabbit monoclonal anti-DAZL antibody (Abcam, cat. no. ab215718; RRID: AB_2893177) • Mouse monoclonal anti-RFP (DsRed) antibody (Rockland, cat. no. 00-301-379S; RRID: AB_2611064) • Goat polyclonal anti-OCT4 antibody (Santa Cruz; cat. no. sc-8628; RRID: AB_653551) • Goat polyclonal anti-DDX4 antibody (R&D Systems, cat. no. AF2030; RRID: AB_2277369) • Donkey anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 488 (Thermo Fisher Scientific, cat. no. A-21206; RRID: AB_2535792) ▲ CAutIon Because Alexa Fluor 488 is photosensitive, exposure to light sources should be minimized.

    Article Title: Generation of porcine induced-pluripotent stem cells from Sertoli cells.
    Article Snippet: Accepted Manuscript Generation of porcine induced-pluripotent stem cells from Sertoli cells Piyathip Setthawong, Praopilas Phakdeedindan, Narong Tiptanavattana, Sasitorn Rungarunlert, Mongkol Techakumphu, Theerawat Tharasanit PII: S0093-691X(18)31094-X DOI: 10.1016/j.theriogenology.2018.12.033 Reference: THE 14825 To appear in: Theriogenology Received Date: 15 May 2018 Accepted Date: 20 December 2018 Please cite this article as: Piyathip Setthawong, Praopilas Phakdeedindan, Narong Tiptanavattana, Sasitorn Rungarunlert, Mongkol Techakumphu, Theerawat Tharasanit, Generation of porcine induced-pluripotent stem cells from Sertoli cells, (2018), doi: 10.1016/j.Theriogenology theriogenology.2018.12.033 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

    Generated:

    Article Title: Reversible Disruption of Specific Transcription Factor-DNA Interactions Using CRISPR/Cas9
    Article Snippet: A goat polyclonal anti-Oct4 antibody (N19, sc-8628, Santa Cruz Biotechnology) was used to pull-down Oct4, a rabbit polyclonal anti-HA tag (ab9110, Abcam) was used to pull down dCas9 tagged with HA and mCherry, and a purified polyclonal rabbit anti-Nanog (Nanog Antibody, A300–397A, Bethyl) was used to pull down Nanog.

    Article Title: Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids.
    Article Snippet: • Sucrose (Sigma-Aldrich, cat. no. S7903) • Optimal cutting temperature embedding cryo-embedding matrix (OCT; Thermo Fisher Scientific, cat. no. 12678646) • Dry ice • Triton X-100 (Sigma-Aldrich, cat. no. 11332481001) • Rabbit monoclonal anti-DAZL antibody (Abcam, cat. no. ab215718; RRID: AB_2893177) • Mouse monoclonal anti-RFP (DsRed) antibody (Rockland, cat. no. 00-301-379S; RRID: AB_2611064) • Goat polyclonal anti-OCT4 antibody (Santa Cruz; cat. no. sc-8628; RRID: AB_653551) • Goat polyclonal anti-DDX4 antibody (R&D Systems, cat. no. AF2030; RRID: AB_2277369) • Donkey anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 488 (Thermo Fisher Scientific, cat. no. A-21206; RRID: AB_2535792) ▲ CAutIon Because Alexa Fluor 488 is photosensitive, exposure to light sources should be minimized.

    Article Title: Generation of porcine induced-pluripotent stem cells from Sertoli cells.
    Article Snippet: Accepted Manuscript Generation of porcine induced-pluripotent stem cells from Sertoli cells Piyathip Setthawong, Praopilas Phakdeedindan, Narong Tiptanavattana, Sasitorn Rungarunlert, Mongkol Techakumphu, Theerawat Tharasanit PII: S0093-691X(18)31094-X DOI: 10.1016/j.theriogenology.2018.12.033 Reference: THE 14825 To appear in: Theriogenology Received Date: 15 May 2018 Accepted Date: 20 December 2018 Please cite this article as: Piyathip Setthawong, Praopilas Phakdeedindan, Narong Tiptanavattana, Sasitorn Rungarunlert, Mongkol Techakumphu, Theerawat Tharasanit, Generation of porcine induced-pluripotent stem cells from Sertoli cells, (2018), doi: 10.1016/j.Theriogenology theriogenology.2018.12.033 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

    Construct:

    Article Title: Reversible Disruption of Specific Transcription Factor-DNA Interactions Using CRISPR/Cas9
    Article Snippet: A goat polyclonal anti-Oct4 antibody (N19, sc-8628, Santa Cruz Biotechnology) was used to pull-down Oct4, a rabbit polyclonal anti-HA tag (ab9110, Abcam) was used to pull down dCas9 tagged with HA and mCherry, and a purified polyclonal rabbit anti-Nanog (Nanog Antibody, A300–397A, Bethyl) was used to pull down Nanog.

    Article Title: Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids.
    Article Snippet: • Sucrose (Sigma-Aldrich, cat. no. S7903) • Optimal cutting temperature embedding cryo-embedding matrix (OCT; Thermo Fisher Scientific, cat. no. 12678646) • Dry ice • Triton X-100 (Sigma-Aldrich, cat. no. 11332481001) • Rabbit monoclonal anti-DAZL antibody (Abcam, cat. no. ab215718; RRID: AB_2893177) • Mouse monoclonal anti-RFP (DsRed) antibody (Rockland, cat. no. 00-301-379S; RRID: AB_2611064) • Goat polyclonal anti-OCT4 antibody (Santa Cruz; cat. no. sc-8628; RRID: AB_653551) • Goat polyclonal anti-DDX4 antibody (R&D Systems, cat. no. AF2030; RRID: AB_2277369) • Donkey anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 488 (Thermo Fisher Scientific, cat. no. A-21206; RRID: AB_2535792) ▲ CAutIon Because Alexa Fluor 488 is photosensitive, exposure to light sources should be minimized.

    Article Title: Generation of porcine induced-pluripotent stem cells from Sertoli cells.
    Article Snippet: Accepted Manuscript Generation of porcine induced-pluripotent stem cells from Sertoli cells Piyathip Setthawong, Praopilas Phakdeedindan, Narong Tiptanavattana, Sasitorn Rungarunlert, Mongkol Techakumphu, Theerawat Tharasanit PII: S0093-691X(18)31094-X DOI: 10.1016/j.theriogenology.2018.12.033 Reference: THE 14825 To appear in: Theriogenology Received Date: 15 May 2018 Accepted Date: 20 December 2018 Please cite this article as: Piyathip Setthawong, Praopilas Phakdeedindan, Narong Tiptanavattana, Sasitorn Rungarunlert, Mongkol Techakumphu, Theerawat Tharasanit, Generation of porcine induced-pluripotent stem cells from Sertoli cells, (2018), doi: 10.1016/j.Theriogenology theriogenology.2018.12.033 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

    Expressing:

    Article Title: Reversible Disruption of Specific Transcription Factor-DNA Interactions Using CRISPR/Cas9
    Article Snippet: A goat polyclonal anti-Oct4 antibody (N19, sc-8628, Santa Cruz Biotechnology) was used to pull-down Oct4, a rabbit polyclonal anti-HA tag (ab9110, Abcam) was used to pull down dCas9 tagged with HA and mCherry, and a purified polyclonal rabbit anti-Nanog (Nanog Antibody, A300–397A, Bethyl) was used to pull down Nanog.

    Article Title: Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids.
    Article Snippet: • Sucrose (Sigma-Aldrich, cat. no. S7903) • Optimal cutting temperature embedding cryo-embedding matrix (OCT; Thermo Fisher Scientific, cat. no. 12678646) • Dry ice • Triton X-100 (Sigma-Aldrich, cat. no. 11332481001) • Rabbit monoclonal anti-DAZL antibody (Abcam, cat. no. ab215718; RRID: AB_2893177) • Mouse monoclonal anti-RFP (DsRed) antibody (Rockland, cat. no. 00-301-379S; RRID: AB_2611064) • Goat polyclonal anti-OCT4 antibody (Santa Cruz; cat. no. sc-8628; RRID: AB_653551) • Goat polyclonal anti-DDX4 antibody (R&D Systems, cat. no. AF2030; RRID: AB_2277369) • Donkey anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 488 (Thermo Fisher Scientific, cat. no. A-21206; RRID: AB_2535792) ▲ CAutIon Because Alexa Fluor 488 is photosensitive, exposure to light sources should be minimized.

    Article Title: Generation of porcine induced-pluripotent stem cells from Sertoli cells.
    Article Snippet: Accepted Manuscript Generation of porcine induced-pluripotent stem cells from Sertoli cells Piyathip Setthawong, Praopilas Phakdeedindan, Narong Tiptanavattana, Sasitorn Rungarunlert, Mongkol Techakumphu, Theerawat Tharasanit PII: S0093-691X(18)31094-X DOI: 10.1016/j.theriogenology.2018.12.033 Reference: THE 14825 To appear in: Theriogenology Received Date: 15 May 2018 Accepted Date: 20 December 2018 Please cite this article as: Piyathip Setthawong, Praopilas Phakdeedindan, Narong Tiptanavattana, Sasitorn Rungarunlert, Mongkol Techakumphu, Theerawat Tharasanit, Generation of porcine induced-pluripotent stem cells from Sertoli cells, (2018), doi: 10.1016/j.Theriogenology theriogenology.2018.12.033 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

    Plasmid Preparation:

    Article Title: Reversible Disruption of Specific Transcription Factor-DNA Interactions Using CRISPR/Cas9
    Article Snippet: A goat polyclonal anti-Oct4 antibody (N19, sc-8628, Santa Cruz Biotechnology) was used to pull-down Oct4, a rabbit polyclonal anti-HA tag (ab9110, Abcam) was used to pull down dCas9 tagged with HA and mCherry, and a purified polyclonal rabbit anti-Nanog (Nanog Antibody, A300–397A, Bethyl) was used to pull down Nanog.

    Article Title: Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids.
    Article Snippet: • Sucrose (Sigma-Aldrich, cat. no. S7903) • Optimal cutting temperature embedding cryo-embedding matrix (OCT; Thermo Fisher Scientific, cat. no. 12678646) • Dry ice • Triton X-100 (Sigma-Aldrich, cat. no. 11332481001) • Rabbit monoclonal anti-DAZL antibody (Abcam, cat. no. ab215718; RRID: AB_2893177) • Mouse monoclonal anti-RFP (DsRed) antibody (Rockland, cat. no. 00-301-379S; RRID: AB_2611064) • Goat polyclonal anti-OCT4 antibody (Santa Cruz; cat. no. sc-8628; RRID: AB_653551) • Goat polyclonal anti-DDX4 antibody (R&D Systems, cat. no. AF2030; RRID: AB_2277369) • Donkey anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 488 (Thermo Fisher Scientific, cat. no. A-21206; RRID: AB_2535792) ▲ CAutIon Because Alexa Fluor 488 is photosensitive, exposure to light sources should be minimized.

    Article Title: Generation of porcine induced-pluripotent stem cells from Sertoli cells.
    Article Snippet: Accepted Manuscript Generation of porcine induced-pluripotent stem cells from Sertoli cells Piyathip Setthawong, Praopilas Phakdeedindan, Narong Tiptanavattana, Sasitorn Rungarunlert, Mongkol Techakumphu, Theerawat Tharasanit PII: S0093-691X(18)31094-X DOI: 10.1016/j.theriogenology.2018.12.033 Reference: THE 14825 To appear in: Theriogenology Received Date: 15 May 2018 Accepted Date: 20 December 2018 Please cite this article as: Piyathip Setthawong, Praopilas Phakdeedindan, Narong Tiptanavattana, Sasitorn Rungarunlert, Mongkol Techakumphu, Theerawat Tharasanit, Generation of porcine induced-pluripotent stem cells from Sertoli cells, (2018), doi: 10.1016/j.Theriogenology theriogenology.2018.12.033 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.



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    Image Search Results


    (A) Top: a diagram ofthe Utf1 locuswith the genomic region of interest shown below indicated by the orange box. Bottom: Oct4 ChlP-seq data near Utf1.Also shown are sgRNA (red) and qPCR primer sequences (blue). The red box denotes open chromatin identified by ENCODE DNase-seq (Experiment number: ENCSR000CMW).

    Journal: Molecular cell

    Article Title: Reversible Disruption of Specific Transcription Factor-DNA Interactions Using CRISPR/Cas9

    doi: 10.1016/j.molcel.2019.04.011

    Figure Lengend Snippet: (A) Top: a diagram ofthe Utf1 locuswith the genomic region of interest shown below indicated by the orange box. Bottom: Oct4 ChlP-seq data near Utf1.Also shown are sgRNA (red) and qPCR primer sequences (blue). The red box denotes open chromatin identified by ENCODE DNase-seq (Experiment number: ENCSR000CMW).

    Article Snippet: A goat polyclonal anti-Oct4 antibody (N19, sc-8628, Santa Cruz Biotechnology) was used to pull-down Oct4, a rabbit polyclonal anti-HA tag (ab9110, Abcam) was used to pull down dCas9 tagged with HA and mCherry, and a purified polyclonal rabbit anti-Nanog (Nanog Antibody, A300–397A, Bethyl) was used to pull down Nanog.

    Techniques:

    (A) Top: a diagram of the Pax6 locus with the genomic region shown below indicated by the orange box. Bottom: ChIP-seq data for Oct4 show the chromosomal position of an Oct4 peak in the regulatory region of the Pax6 gene. Also shown is the position of the targeting sgRNA (red). The red box denotes open chromatin identified by ENCODE DNase-seq (Experiment number: ENCSR000CMW).

    Journal: Molecular cell

    Article Title: Reversible Disruption of Specific Transcription Factor-DNA Interactions Using CRISPR/Cas9

    doi: 10.1016/j.molcel.2019.04.011

    Figure Lengend Snippet: (A) Top: a diagram of the Pax6 locus with the genomic region shown below indicated by the orange box. Bottom: ChIP-seq data for Oct4 show the chromosomal position of an Oct4 peak in the regulatory region of the Pax6 gene. Also shown is the position of the targeting sgRNA (red). The red box denotes open chromatin identified by ENCODE DNase-seq (Experiment number: ENCSR000CMW).

    Article Snippet: A goat polyclonal anti-Oct4 antibody (N19, sc-8628, Santa Cruz Biotechnology) was used to pull-down Oct4, a rabbit polyclonal anti-HA tag (ab9110, Abcam) was used to pull down dCas9 tagged with HA and mCherry, and a purified polyclonal rabbit anti-Nanog (Nanog Antibody, A300–397A, Bethyl) was used to pull down Nanog.

    Techniques: ChIP-sequencing

    (A) The sequence logo of Oct4:Sox2 binding sites ± 30 bp flanking regions shows that the flanking regions are random. The height of base pairs signifies the degree of conservation for each position.

    Journal: Molecular cell

    Article Title: Reversible Disruption of Specific Transcription Factor-DNA Interactions Using CRISPR/Cas9

    doi: 10.1016/j.molcel.2019.04.011

    Figure Lengend Snippet: (A) The sequence logo of Oct4:Sox2 binding sites ± 30 bp flanking regions shows that the flanking regions are random. The height of base pairs signifies the degree of conservation for each position.

    Article Snippet: A goat polyclonal anti-Oct4 antibody (N19, sc-8628, Santa Cruz Biotechnology) was used to pull-down Oct4, a rabbit polyclonal anti-HA tag (ab9110, Abcam) was used to pull down dCas9 tagged with HA and mCherry, and a purified polyclonal rabbit anti-Nanog (Nanog Antibody, A300–397A, Bethyl) was used to pull down Nanog.

    Techniques: Sequencing, Binding Assay

    KEY RESOURCES TABLE

    Journal: Molecular cell

    Article Title: Reversible Disruption of Specific Transcription Factor-DNA Interactions Using CRISPR/Cas9

    doi: 10.1016/j.molcel.2019.04.011

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: A goat polyclonal anti-Oct4 antibody (N19, sc-8628, Santa Cruz Biotechnology) was used to pull-down Oct4, a rabbit polyclonal anti-HA tag (ab9110, Abcam) was used to pull down dCas9 tagged with HA and mCherry, and a purified polyclonal rabbit anti-Nanog (Nanog Antibody, A300–397A, Bethyl) was used to pull down Nanog.

    Techniques: Virus, Recombinant, Purification, Chromatin Immunoprecipitation, Cloning, Generated, Construct, Expressing, Plasmid Preparation